A mariner-based transposition system for Listeria monocytogenes

被引：45

作者：

Cao, Min ^{[1
]}

Bitar, Alan Pavinski ^{[1
]}

Marquis, Helene ^{[1
]}

机构：

[1] Cornell Univ, Dept Microbiol & Immunol, Ithaca, NY 14853 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 08期

关键词：

D O I：

10.1128/AEM.02844-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes.

引用

页码：2758 / 2761

页数：4

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