The influence of ADAR1's regulation on lymphocyte cell function during rejection

被引：6

作者：

Cai, Lei ^{[1
]}

Li, Yan ^{[2
]}

Liu, Feng ^{[3
]}

Zhang, Wei ^{[1
]}

Huo, Binliang ^{[1
,4
]}

Zheng, Wei ^{[1
,4
]}

Ding, Rui ^{[1
]}

Guo, Jiyuan ^{[5
]}

Zhao, Qingchuan ^{[1
]}

Dou, Kefeng ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Dept Hepatobiliary Surg, Xijing Hosp, Xian 710032, Peoples R China

[2] Fourth Mil Med Univ, Dept Anesthesia, Xijing Hosp, Xian 710032, Peoples R China

[3] Shanghai Pudong New Area Gongli Hosp, Dept Orthopaed, Shanghai 200135, Peoples R China

[4] Shaanxi Prov Peoples Hosp, Dept Gen Surg, Xian 710068, Peoples R China

[5] Xian Childrens Hosp, Dept Emergency, Xian 710002, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 06期

关键词：

ADAR1; Mixed lymphocyte culture; T primary lymphocyte; Acute rejection; RNAi; DOUBLE-STRANDED-RNA; ACUTE ALLOGRAFT-REJECTION; DNA-DAMAGE CHECKPOINT; TRANSPLANT RECIPIENTS; ADENOSINE-DEAMINASE; EDITING ENZYME; T-CELLS; INTERFERON; JAK1; GENE;

D O I：

10.1007/s11033-009-9804-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNA editing adenosine deaminase gene (ADAR1) expression is wide-spread in lymphocytes. We explore the mechanism of ADAR1 regulation on the function of T primary lymphocytes when rejection occurs by knock-down the expression of ADAR1 in mouse T primary lymphocytes in mixed lymphocyte cultures. The changes of cell proliferation, the expression of ADAR1 and the cell cycle related genes cyclin D1 and A1, cell cycle and apoptosis analysis and mouse gene expression profiles was evaluated. We found that treatment with ADAR1-specific siRNA inhibited allogenic antigen stimulated T cell proliferation, arrested T cell cycle at G(0)/G(1) phases and promoted T cell apoptosis, which was associated with down-regulation of some related key genes transcription. These findings suggest that ADAR1 is essential for the maintenance of function of T lymphocytes during acute rejection. The mechanism underlying ADAR1's action might include editing of a currently unknown substrate and interacting with other proteins.

引用

页码：2703 / 2709

页数：7

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