A myosin I isoform in the nucleus

被引：198

作者：

Pestic-Dragovich, L

Stojiljkovic, L

Philimonenko, AA

Nowak, G

Ke, Y

Settlage, RE

Shabanowitz, J

Hunt, DF

Hozak, P

de Lanerolle, P ^{[1
]}

机构：

[1] Univ Illinois, Dept Physiol & Biophys, Chicago, IL 60612 USA

[2] Acad Sci Czech Republ, Inst Expt Med, Dept Cell Ultrastruct & Mol Biol, Prague, Czech Republic

[3] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA

[4] Univ Virginia, Dept Pathol, Charlottesville, VA 22901 USA

来源：

SCIENCE | 2000年 / 290卷 / 5490期

关键词：

D O I：

10.1126/science.290.5490.337

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A nuclear isoform of myosin I beta that contains a unique 16-amino acid aminoterminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I beta colocalized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I beta appears to be in a complex with RNA polymerase II and may affect transcription.

引用

页码：337 / 341

页数：5

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