Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus

被引:20
作者
Harrington, DJ
Greated, JS
Chanter, N
Sutcliffe, IC
机构
[1] Univ Sunderland, Sch Sci, Sunderland SR2 3SD, Durham, England
[2] Anim Hlth Trust, Newmarket, Suffolk, England
基金
英国惠康基金;
关键词
D O I
10.1128/IAI.68.10.6048-6051.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Streptococcus equi and Streptococcus zooepidemicus are major etiological agents of upper and lower airway disease in horses. Despite the considerable animal suffering and economic burden associated with these diseases, the factors that contribute to the virulence of these equine pathogens have not been extensively investigated. Here we demonstrate the presence of a homologue of the Streptococcus pneumoniae PsaA protein in both of these equine pathogens. Inhibition of signal peptide processing by the antibiotic globomycin confirmed the lipoprotein nature of the mature proteins, and surface exposure was confirmed by their release from intact cells by mild trypsinolysis.
引用
收藏
页码:6048 / 6051
页数:4
相关论文
共 37 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   MOLECULAR-IDENTIFICATION OF AN ABC TRANSPORTER COMPLEX FOR MANGANESE - ANALYSIS OF A CYANOBACTERIAL MUTANT STRAIN IMPAIRED IN THE PHOTOSYNTHETIC OXYGEN EVOLUTION PROCESS [J].
BARTSEVICH, VV ;
PAKRASI, HB .
EMBO JOURNAL, 1995, 14 (09) :1845-1853
[3]   An ABC transporter system of Yersinia pestis allows utilization of chelated iron by Escherichia coli SAB11 [J].
Bearden, SW ;
Staggs, TM ;
Perry, RD .
JOURNAL OF BACTERIOLOGY, 1998, 180 (05) :1135-1147
[4]   Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae [J].
Berry, AM ;
Paton, JC .
INFECTION AND IMMUNITY, 1996, 64 (12) :5255-5262
[5]   FIMA, A MAJOR VIRULENCE FACTOR ASSOCIATED WITH STREPTOCOCCUS-PARASANGUIS ENDOCARDITIS [J].
BURNETTECURLEY, D ;
WELLS, V ;
VISCOUNT, H ;
MUNRO, CL ;
FENNO, JC ;
FIVESTAYLOR, P ;
MACRINA, FL .
INFECTION AND IMMUNITY, 1995, 63 (12) :4669-4674
[6]   Recombinant hyaluronate associated protein as a protective immunogen against Streptococcus equi and Streptococcus zooepidemicus challenge in mice [J].
Chanter, N ;
Ward, CL ;
Talbot, NC ;
Flanagan, JA ;
Binns, M ;
Houghton, SB ;
Smith, KC ;
Mumford, JA .
MICROBIAL PATHOGENESIS, 1999, 27 (03) :133-143
[7]   Streptococci and enterococci as animal pathogens [J].
Chanter, N .
JOURNAL OF APPLIED MICROBIOLOGY, 1997, 83 :S100-S109
[8]   EQUINE STRANGLES MODELED IN MICE [J].
CHANTER, N ;
SMITH, KC ;
MUMFORD, JA .
VETERINARY MICROBIOLOGY, 1995, 43 (2-3) :209-218
[9]   Characterization of the Lancefield group C Streptococcus 16S-23S RNA gene intergenic spacer and its potential for identification and sub-specific typing [J].
Chanter, N ;
Collin, N ;
Holmes, N ;
Binns, M ;
Mumford, J .
EPIDEMIOLOGY AND INFECTION, 1997, 118 (02) :125-135
[10]   Molecular cloning of a 32-kilodalton lipoprotein component of a novel iron-regulated Staphylococcus epidermidis ABC transporter [J].
Cockayne, A ;
Hill, PJ ;
Powell, NBL ;
Bishop, K ;
Sims, C ;
Williams, P .
INFECTION AND IMMUNITY, 1998, 66 (08) :3767-3774