Simultaneous measurement of 8-oxo-2′-deoxyguanosine and 8-oxo-2′-deoxyadenosine by HPLC-MS/MS

被引：34

作者：

Podmore, ID

Cooper, D

Evans, MD

Wood, M

Lunec, J

机构：

[1] Univ Leicester, Leicester Royal Infirm, Univ Hosp Leicester NHS Trust, Div Chem Pathol,Oxidat Stress Grp, Leicester LE2 7LX, Leics, England

[2] Micromass UK Ltd, Clin Applicat Grp, Manchester M23 9LZ, Lancs, England

[3] Micromass UK Ltd, Applicat Res Lab, Manchester M23 9LZ, Lancs, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 277卷 / 03期

关键词：

8-oxo-2 '-deoxyguanosine; 8-oxo-2 '-deoxyadenosine; HPLC-MS/MS; biomarkers; DNA lesions; oxidative stress;

D O I：

10.1006/bbrc.2000.3752

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay with high selectivity and sensitivity has been developed which, for the first time, allows quantitative, simultaneous measurement in DNA of both 8-oxo-2'-deoxyguanosine (8-oxodG) and 8-oxo-2'-deoxyadenosine (8-oxodA)-important biomarkers of oxidative DNA damage in vivo. Using reversed-phase HPLC coupled to electrospray tandem mass spectrometry (HPLC-MS/MS) in multiple reaction monitoring (MRM) mode it was possible to detect background levels of these lesions in commercially available calf thymus DNA (85 +/- 3 and 7.1 +/- 0.2 per 10(6) DNA bases for 8-oxodG and 8-oxodA respectively; n = 3). Levels of 8-oxodG determined by HPLC coupled to an electrochemical detection system (HPLC-EC) were found to be similar (75 +/- 6 per 10(6) DNA bases; n = 3) to those obtained using tandem mass spectrometry. (C) 2000 Academic Press.

引用

页码：764 / 770

页数：7

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