Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

被引：81

作者：

Burkart, MD

Izumi, M

Chapman, E

Lin, CH

Wong, CH

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2000年 / 65卷 / 18期

关键词：

D O I：

10.1021/jo000266o

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyltransferases.

引用

页码：5565 / 5574

页数：10

共 45 条

[1] SYNTHESIS OF ACTIVE SULPHATE (ADENOSINE 3'-PHOSPHATE 5'-SULPHATOPHOSPHATE) [J].