Typing and subtyping of Clostridium difficile isolates by using multiple-locus variable-number tandem-repeat analysis

被引:118
作者
van den Berg, Renate J.
Schaap, Inge
Templeton, Kate E.
Klaassen, Corne H. W.
Kuijper, Ed J.
机构
[1] Leiden Univ, Dept Med Microbiol, Ctr Infect Dis, NL-2300 RC Leiden, Netherlands
[2] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands
关键词
D O I
10.1128/JCM.02023-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Using the genomic sequence of Clostridium difficile strain 630, we developed multiple-locus variable-number tandem-repeat analysis (MLVA) with automated fragment analysis and multicolored capillary electrophoresis as a typing method for C difficile. All reference strains, representing 31 serogroups, 25 toxinotypes, and 7 known subtypes of PCR ribotype 001, could be discriminated from each other. Application of MLVA to 28 isolates from 7 outbreaks due to the emerging hypervirulent PCR ribotype 027-pulsed-field gel electrophoresis type NAP1 resulted in recognition of 13 clusters. Additionally, 29 toxin A-negative, toxin B-positive isolates belonging to PCR ribotype 017 from eight different countries revealed eight country-specific clusters. MLVA is a highly discriminatory genotyping method and a new tool for subtyping of newly emerging variants of C. difficile.
引用
收藏
页码:1024 / 1028
页数:5
相关论文
共 19 条
[1]  
al-Barrak A, 1999, Can Commun Dis Rep, V25, P65
[2]   Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea [J].
Alfa, MJ ;
Kabani, A ;
Lyerly, D ;
Moncrief, S ;
Neville, LM ;
Al-Barrak, A ;
Harding, GKH ;
Dyck, B ;
Olekson, K ;
Embil, JM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) :2706-2714
[3]   Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing [J].
Bidet, P ;
Barbut, F ;
Lalande, V ;
Burghoffer, B ;
Petit, JC .
FEMS MICROBIOLOGY LETTERS, 1999, 175 (02) :261-266
[4]   Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates [J].
de Valk, HA ;
Meis, JFGM ;
Curfs, IM ;
Muehlethaler, K ;
Mouton, JW ;
Klaassen, CHW .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4112-4120
[5]   Molecular epidemiology of endemic Clostridium difficile infection and the significance of subtypes of the United Kingdom epidemic strain (PCR ribotype 1) [J].
Fawley, WN ;
Parnell, P ;
Verity, P ;
Freeman, J ;
Wilcox, MH .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (06) :2685-2696
[6]  
*HLTH PROT AG, 2006, CLOST DIFF FIND REC
[7]   Emergence of Clostridium difficile-associated disease in North America and Europe [J].
Kuijper, E. J. ;
Coignard, B. ;
Tull, P. .
CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 :2-18
[8]  
Kuijper EJ, 2006, EMERG INFECT DIS, V12, P827
[9]   Nosocomial outbreak of Clostridium difficile-associated diarrhoea due to a clindamycin-resistant enterotoxin A-negative strain [J].
Kuijper, EJ ;
de Weerdt, J ;
Kato, H ;
Kato, N ;
van Dam, AP ;
van der Vorm, ER ;
Weel, J ;
van Rheenen, C ;
Dankert, J .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2001, 20 (08) :528-534
[10]   Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria [J].
Lindstedt, BA .
ELECTROPHORESIS, 2005, 26 (13) :2567-2582