Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples

被引：12

作者：

Akens, MK

Holznagel, E

Franchini, M

Bracher, V

机构：

[1] Univ Zurich, Dept Vet Internal Med, CH-8057 Zurich, Switzerland

[2] Univ Bern, Inst Vet Pathol, Bern, Switzerland

[3] Univ Zurich, Inst Vet Physiol, Zurich, Switzerland

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1997年 / 58卷 / 3-4期

关键词：

equine; lymphocytes; flow cytometry; immunophenotyping;

D O I：

10.1016/S0165-2427(97)00044-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque((R)) separation, prior to FAGS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods. (C) 1997 Elsevier Science B.V.

引用

收藏

页码：231 / 237

页数：7

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