Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis

被引：70

作者：

Cunillera, N ^{[1
]}

Arró, M ^{[1
]}

Fóres, O ^{[1
]}

Manzano, D ^{[1
]}

Ferrer, A ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

来源：

FEBS LETTERS | 2000年 / 477卷 / 03期

关键词：

cis-prenyltransferase; dehydrodolichyl diphosphate synthase; isoprenoid; Rer2p; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(00)01798-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme dehydrodolichyl diphosphate (dedol-PP) synthase is a cis-prenyltransferase that catalyzes the synthesis of dedol-PP, the long-chain polyprenyl diphosphate used as a precursor for the synthesis of dolichyl phosphate, Here we report the cloning and characterization of a cDNA from Arabidopsis thaliana encoding dedol-PP synthase, The identity of the cloned enzyme was confirmed by functional complementation of a yeast mutant strain defective in dedol-PP synthase activity together with the detection of high levels of dedol-PP synthase activity in the transformed yeast mutant, The A. thaliana dedol-PP synthase mRNA was detected at high levels in roots but was hardly detected in flowers, leaves, stems and in A, thaliana suspension-cultured cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：170 / 174

页数：5

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