Extensive clonality in the endangered shrub Haloragodendron lucasii (Haloragaceae) revealed by allozymes and RAPDs

被引:96
作者
Sydes, MA [1 ]
Peakall, R [1 ]
机构
[1] Australian Natl Univ, Div Bot & Zool, Canberra, ACT 0200, Australia
关键词
allozyme; clonality; clone identification; conservation management; endangered species; RAPD;
D O I
10.1046/j.1365-294x.1998.00314.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The occurrence of clonality in threatened plants can have important implications for their conservation. In this study, allozymes and RAPDs were used to determine the extent of clonality in the endangered shrub Haloragodendron lucasii (Haloragaceae), which is known from only four sites within an 8 km range. Allozyme markers identified only six multilocus genotypes among the 53 ramets sampled across the four sites, although a total of 54 different genotypes were possible with the three polymorphic allozyme loci detected. The polymorphic bands detected in the RAPD analysis were capable of producing 2(46) genotypes, but again only six multilocus genotypes were delineated. The allozyme and RAPD data were congruent at three of the four sites. At the fourth site two genotypes were detected by each marker; however, once combined, three multilocus genotypes were observed. The probabilities that the observed number of replicates of each combined allozyme and RAPD genotype could be generated by sexual reproduction were less than 10(-18), leaving little doubt that clonality is the explanation for the observed patterns of genotypes. The genetic conclusions are supported by root excavations which show potential for vegetative reproduction and the observation of no sexual reproduction in the species. The recognition of extensive clonality in H. lucasii has had immediate implications for the conservation management of the species and resulted in changes to the management priorities for the species. Thus it is clear that appropriate genetic studies can play an important role in the management of threatened species.
引用
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页码:87 / 93
页数:7
相关论文
共 21 条
[1]  
AULD TD, 1991, GERMINATION RARE PLA
[2]   SPATIAL AND GENETIC-STRUCTURE OF 2 SANDHILLS OAKS - QUERCUS LAEVIS AND QUERCUS-MARGARETTA (FAGACEAE) [J].
BERG, EE ;
HAMRICK, JL .
AMERICAN JOURNAL OF BOTANY, 1994, 81 (01) :7-14
[3]  
Briggs JD, 1996, RARE THREATENED AUST
[4]  
COATES DJ, 1988, AUSTR J BOTANY, V36, P22
[5]  
Doyle JJ, 1987, PHYTOCHEMISTRY B, V19, P11
[6]   PATTERNS OF GENOTYPIC DIVERSITY IN CLONAL PLANT-SPECIES [J].
ELLSTRAND, NC ;
ROOSE, ML .
AMERICAN JOURNAL OF BOTANY, 1987, 74 (01) :123-131
[7]  
Gardner C., 1991, FLORA NEW S WALES, V2, P442
[8]   GENETIC DIVERSITY IN A THREATENED WETLAND SPECIES, HELONIAS-BULLATA (LILIACEAE) [J].
GODT, MJW ;
HAMRICK, JL ;
BRATTON, S .
CONSERVATION BIOLOGY, 1995, 9 (03) :596-604
[9]   THE INTRUSION OF CLONAL GROWTH-PATTERNS ON PLANT-BREEDING SYSTEMS [J].
HANDEL, SN .
AMERICAN NATURALIST, 1985, 125 (03) :367-384
[10]   DNA fingerprinting supports notions of clonality in a rare mallee, Eucalyptus argutifolia [J].
Kennington, WJ ;
Waycott, M ;
James, SH .
MOLECULAR ECOLOGY, 1996, 5 (05) :693-696