Continuous on-line concentration based on dynamic pH junction for trimethoprim and sulfamethoxazole by microfluidic capillary electrophoresis combined with flow injection analysis system

被引：39

作者：

Fan, LY ^{[1
]}

Liu, LH ^{[1
]}

Chen, HL ^{[1
]}

Chen, XG ^{[1
]}

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Gansu, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1062卷 / 01期

基金：

中国国家自然科学基金;

关键词：

microfluidic capillary electrophoresis; flow injection; dynamic pH junction; trimethoprim; sulfamethoxazole;

D O I：

10.1016/j.chroma.2004.11.035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel, rapid and continuous on-line concentration approach based on dynamic pH junction for the analysis of trimethoprim, (TMP) and sulfamethoxazole (SMZ) by microfluidic capillary electrophoresis (CE) combined with flow injection analysis is developed in this paper. Stacking is due to decreases in the velocity of analytes when migrating from the low-pH sample zone (sample was dissolved in 50 MM HCl) to a relatively high-pH buffer (30 mM phosphate buffer, pH 8.5) filled in the capillary. This results in 2.9-4.7-fold improvement in concentration sensitivity relative to conventional capillary electrophoresis methods. The separation could be achieved within 2 min and sample throughput rate can reach up to 38 h(-1). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：133 / 137

页数：5

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