Targeted comparative proteomics by liquid chromatography-tandem Fourier ion cyclotron resonance mass spectrometry

被引：38

作者：

Masselon, C ^{[1
]}

Pasa-Tolic, L ^{[1
]}

Tolic, N ^{[1
]}

Anderson, GA ^{[1
]}

Bogdanov, B ^{[1
]}

Vilkov, AN ^{[1
]}

Shen, YF ^{[1
]}

Zhao, R ^{[1
]}

Oian, WJ ^{[1
]}

Lipton, MS ^{[1
]}

Camp, DG ^{[1
]}

Smith, RD ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 02期

关键词：

D O I：

10.1021/ac049043e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In proteomics, effective methods are needed for identifying the relatively limited subset of proteins displaying significant changes in abundance between two samples. One way to accomplish this task is to target for identification by MS/MS only the "interesting" proteins based on the abundance ratio of isotopically labeled pairs of peptides. We have developed the software and hardware tools for online LC-FTICR MS/MS studies in which a set of initially unidentified peptides from a proteome analysis can be selected for identification based on their distinctive changes in abundance following a "perturbation". We report here the validation of this method using a mixture of standard proteins combined in different ratios after isotopic labeling. We also demonstrate the application of this method to the identification of Shewanella oneiden-sis peptides/proteins exhibiting differential abundance in suboxic versus aerobic cell cultures.

引用

页码：400 / 406

页数：7

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