Structure of a stereoregular phosphorothioate DNA/RNA duplex

被引：47

作者：

Bachelin, M

Hessler, G

Kurz, G

Hacia, JG

Dervan, PB

Kessler, H

机构：

[1] Tech Univ Munich, Inst Organ Chem & Biochem, D-85747 Garching, Germany

[2] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[3] CALTECH, Beckman Inst, Pasadena, CA 91125 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 04期

关键词：

D O I：

10.1038/nsb0498-271

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular R-p-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-R-p)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.

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页码：271 / 276

页数：6

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