Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections

被引:66
作者
Lind-Brandberg, L
Welinder-Olsson, C
Lagergård, T
Taranger, J
Trollfors, B
Zackrisson, G
机构
[1] Gothenburg Univ, Dept Clin Bacteriol, S-41346 Gothenburg, Sweden
[2] Gothenburg Univ, Dept Med Microbiol & Immunol, Gothenburg, Sweden
[3] Gothenburg Univ, Dept Pediat, Gothenburg, Sweden
关键词
D O I
10.1128/JCM.36.3.679-683.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
PCR, using primers PIp1 and PIp2, was evaluated for the detection of DNA from Bordetella pertussis in bacterial strains and in nasopharyngeal samples from patients with a cough lasting at least 7 days. The assay could detect DNA from 6 CFU of B., pertussis/10 mu l of sample, Results of the PCR assay were compared with those of cultures, a determination of serum antibodies against pertussis toxin and filamentous hemagglutinin, and a clinical evaluation of 2,442 coughing episodes, The overall sensitivity of PCR was 65% (623 of 956), which was higher than the sensitivity of cultures (58%) (P < 0.001), Factors influencing the sensitivity of PCR were the interval between the onset of symptoms and sampling and the vaccination status of the patient, The specificity of PCR was 98% (1,451 of 1,486), The positive and negative predictive values were 95 and 81%, respectively, Parapertussis PCR, using primers BPPA and BPPZ, was positive in 11 of 18 culture-positive cases and was confirmed by serology in another 4 cases, In conclusion, PCR is a valuable complement to cultures and can probably replace cultures for diagnosis of B., pertussis and Bordetella parapertussis infections.
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页码:679 / 683
页数:5
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