Separation and determination of phospholipids in biological samples by high-performance liquid chromatography

被引：11

作者：

Wang, FJ ^{[1
]}

Zhao, Y ^{[1
]}

Wang, P ^{[1
]}

机构：

[1] Third Mil Med Univ, Southwest Hosp, Inst Burn Res, Chongqing 400038, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2003年 / 41卷 / 03期

关键词：

D O I：

10.1093/chromsci/41.3.142

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic high-performance liquid chromatographic method is developed for the determination of phospholipids in biological samples using a pPorasil silica column and a mobile phase of acetonitrile-methanol-85% phosphoric acid (90: 31, v/v/v) at a flow rate of 0.80 mL./min. The effluent is monitored by a UV detector at 203 nm. With the method reported in this paper, phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine in biological samples are separated and detected successfully. The method is simple, rapid, and has excellent precision.

引用

页码：142 / 144

页数：3