High-throughput screening assays for the identification of chemical probes

被引：437

作者：

Inglese, James ^{[1
]}

Johnson, Ronald L. ^{[1
]}

Simeonov, Anton ^{[1
]}

Xia, Menghang ^{[1
]}

Zheng, Wei ^{[1
]}

Austin, Christopher P. ^{[1
]}

Auld, Douglas S. ^{[1
]}

机构：

[1] US Natl Inst Hlth, Chem Genom Ctr, Bethesda, MD 20892 USA

来源：

NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 08期

关键词：

D O I：

10.1038/nchembio.2007.17

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput screening (HTS) assays enable the testing of large numbers of chemical substances for activity in diverse areas of biology. The biological responses measured in HTS assays span isolated biochemical systems containing purified receptors or enzymes to signal transduction pathways and complex networks functioning in cellular environments. This Review addresses factors that need to be considered when implementing assays for HTS and is aimed particularly at investigators new to this field. We discuss assay design strategies, the major detection technologies and examples of HTS assays for common target classes, cellular pathways and simple cellular phenotypes. We conclude with special considerations for configuring sensitive, robust, informative and economically feasible HTS assays.

引用

页码：466 / 479

页数：14

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