Entirely automated quantification of human immunodeficiency virus type 1 (HIV-1) RNA in plasma by using the ultrasensitive COBAS AMPLICOR HIV-1 monitor test and RNA purification on the MagNA pure LC instrument

被引：12

作者：

Hölzl, G ^{[1
]}

Stöcher, M ^{[1
]}

Leb, V ^{[1
]}

Stekel, H ^{[1
]}

Berg, J ^{[1
]}

机构：

[1] Gen Hosp Linz, Inst Lab Med, A-4020 Linz, Austria

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 03期

关键词：

D O I：

10.1128/JCM.41.3.1248-1251.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ultrasensitive COBAS AMPLICOR HIV-1 Monitor test was complemented with automated RNA purification on the MagNA Pure LC instrument. This enabled entirely automated ultrasensitive assessment of viral loads in human immunodeficiency virus type 1 (HIV-1)-infected individuals. The detection limit of the fully automated assay and the viral load measurements in 80 clinical samples were found to be in good agreement with those of the conventional ultrasensitive COBAS AMPLICOR HIV-1 Monitor test. The fully automated assay showed markedly reduced hands-on time and was found to be suitable for the routine assessment of HIV-1 viral loads in a clinical diagnostic laboratory.

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页码：1248 / 1251

页数：4

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