Gene expression profiling in islet biology and diabetes research

被引：18

作者：

Bernal-Mizrachi, E ^{[1
]}

Cras-Méneur, C ^{[1
]}

Ohsugi, M ^{[1
]}

Permutt, MA ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Div Endocrinol Diabet & Metab, St Louis, MO 63110 USA

来源：

DIABETES-METABOLISM RESEARCH AND REVIEWS | 2003年 / 19卷 / 01期

关键词：

cDNA library; SAGE; microarray; gene expression profile; endocrine pancreas consortium;

D O I：

10.1002/dmrr.331

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Following the sequencing of most of the human and mouse genomes, the next task for physicians and scientists will be to assess the relative levels of expression of these genes during development, following exposure to various nutritional and pharmacological conditions, and in disease states such as diabetes and related metabolic disorders. This review provides an overview of the various methodologies available for monitoring global gene expression. Use of cDNA libraries, Expressed Sequence Tag (EST) sequencing projects and databases, differential display (DD), serial analysis of gene expression (SAGE), subtractive cloning, and both cDNA and oligo microarrays are discussed, along with their merits and limitations. The Endocrine Pancreas Consortium http://www.cbil.upenn.edu/EPConDB/ has constructed mouse and human cDNA libraries from adult and various stages of embryonic development of endocrine pancreas. Over 100 000 ESTs have been deposited in public databases, and each clone is available through the IMAGE Consortium. A guide to Internet access is provided for future investigation. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：32 / 42

页数：11

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