Cloning of TC-1 (C8orf4), a novel gene found to be overexpressed in thyroid cancer

被引：51

作者：

Chua, EL ^{[1
]}

Young, L ^{[1
]}

Wu, WM ^{[1
]}

Turtle, JR ^{[1
]}

Dong, QH ^{[1
]}

机构：

[1] Univ Sydney, Dept Med, Sydney, NSW 2006, Australia

来源：

GENOMICS | 2000年 / 69卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/geno.2000.6348

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel gene highly expressed in thyroid cancer, designated TC-1 (thyroid cancer-1), was cloned from suppression subtractive hybridization between papillary thyroid carcinoma and its surrounding normal thyroid tissue. Overexpression of TC-1 in thyroid cancer was confirmed in 15/16 paired samples by RT-PCR and Northern analysis. Ubiquitously expressed in human tissues, the TC-1 sequence showed no homology to any known gene, but matched a cluster of ESTs, After alignment of our sequence with the ESTs, the missing transcription start site was obtained by 5'-RACE and verified by primer extension analysis. The full-length mRNA sequence of 1327 bp has an open reading frame of 321 bp, which encodes a highly conserved protein. Three regulatory motifs were identified at the expected positions within 1 kb of the 5' flanking sequence obtained by genome walking. Using fluorescence in situ hybridization, TC-1 was localized to chromosome 8p11.2. The overexpression of TC-1 in papillary carcinoma suggests that it may have an important role in thyroid carcinogenesis. (C) 2000 Academic Press.

引用

页码：342 / 347

页数：6

共 24 条

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