Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-Like proteins

被引:176
作者
Moore, FL
Jaruzelska, J
Fox, MS
Urano, J
Firpo, MT
Turek, PJ
Dorfman, DM
Pera, RAR [1 ]
机构
[1] Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Urol, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Program Human Genet, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Program Canc Genet, San Francisco, CA 94143 USA
[6] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland
[7] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA
关键词
D O I
10.1073/pnas.0234478100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Early in development, a part of the embryo is set aside to become the germ cell lineage that will ultimately differentiate to form sperm and eggs and transmit genetic information to the next generation. Men with deletions encompassing the Y-chromosome DAZ genes have few or no germ cells but are otherwise healthy, indicating they harbor specific defects in formation or maintenance of germ cells. A DAZ homolog, DAZL (DAZ-Like), is found in diverse organisms, including humans and is required for germ cell development in males and/or females. We identified proteins that interact with DAZ proteins to better understand their function in human germ cells. Here, we show that PUM2, a human homolog of Pumilio, a protein required to maintain germ line stem cells in Drosophila and Caenorhabditis elegans, forms a stable complex with DAZ through the same functional domain required for RNA binding, protein-protein interactions and rescue of Pumilio mutations in flies. We also show that PUM2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells. These data implicate PUM2 as a component of conserved cellular machinery that may be required for germ cell development.
引用
收藏
页码:538 / 543
页数:6
相关论文
共 35 条
[1]   Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos [J].
Asaoka-Taguchi, M ;
Yamada, M ;
Nakamura, A ;
Hanyu, K ;
Kobayashi, S .
NATURE CELL BIOLOGY, 1999, 1 (07) :431-437
[2]  
CHUBB C, 1992, J ANDROL, V13, P312
[3]   A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans [J].
Crittenden, SL ;
Bernstein, DS ;
Bachorik, JL ;
Thompson, BE ;
Gallegos, M ;
Petcherski, AG ;
Moulder, G ;
Barstead, R ;
Wickens, M ;
Kimble, J .
NATURE, 2002, 417 (6889) :660-663
[4]  
Dorfman DM, 1999, HUM REPROD, V14, P2531
[5]   The quaking gene product necessary in embryogenesis and myelination combines features of RNA binding and signal transduction proteins [J].
Ebersole, TA ;
Chen, Q ;
Justice, MJ ;
Artzt, K .
NATURE GENETICS, 1996, 12 (03) :260-265
[6]   Structure of Pumilio reveals similarity between RNA and peptide binding motifs [J].
Edwards, TA ;
Pyle, SE ;
Wharton, RP ;
Aggarwal, AK .
CELL, 2001, 105 (02) :281-289
[7]  
Forbes A, 1998, DEVELOPMENT, V125, P679
[8]  
Houston DW, 1998, DEVELOPMENT, V125, P171
[9]  
Houston DW, 2000, DEVELOPMENT, V127, P447
[10]   Identification of target messenger RNA substrates for the murine deleted in azoospermia-like RNA-binding protein [J].
Jiao, XF ;
Trifillis, P ;
Kiledjian, M .
BIOLOGY OF REPRODUCTION, 2002, 66 (02) :475-485