Quantitative determination of the β-blocker labetalol in pharmaceuticals and human urine by high-performance liquid chromatography with amperometric detection

被引:38
作者
Ceniceros, C [1 ]
Maguregui, MI [1 ]
Jiménez, RM [1 ]
Alonso, RM [1 ]
机构
[1] Univ Basque Country, Fac Ciencias, Dept Quim Anal, E-48080 Bilbao, Spain
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 705卷 / 01期
关键词
labetalol;
D O I
10.1016/S0378-4347(97)00492-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid and simple high-performance liquid chromatographic (HPLC) method with amperometric detection has been developed for the quantitation of labetalol in urine. The chromatography was performed at 30 degrees C using a reversed-phase column with a base deactivated silica stationary support and an alkylamide bonded phase (Supelcosil ABZ+Plus). A 5 mM acetate buffer (pH 4.5)-acetonitrile (70:30, v/v) mixture was employed as the mobile phase, pumped at a flow-rate of 1 ml/min. Sample preparation was carried out using a simple solid-phase extraction (SPE) procedure, and recoveries higher than 85% were achieved. The method was found to be accurate, precise (R.S.D lower than 8%), and sensitive enough (experimental quantitation limit of 20 ng/ml, detection limit 10 ng/ml) to be applied to doping analysis and pharmacokinetic studies in human urine. The method was applied to the determination of labetalol in pharmaceutical formulations and urine samples obtained from a healthy volunteer after the ingestion of a therapeutic dose of the drug, and the results obtained were in agreement with the pharmacokinetic data. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:97 / 103
页数:7
相关论文
共 20 条
  • [1] LABETALOL ANALYSIS IN HUMAN-PLASMA USING LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - APPLICATION TO PHARMACOKINETIC STUDIES
    ABERNETHY, DR
    TODD, EL
    EGAN, JL
    CARRUM, G
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1986, 9 (10): : 2153 - 2164
  • [2] ALI SA, 1992, J CHEM SOC PAKISTAN, V14, P257
  • [3] COMBINED USE OF AN AUTOMATED SAMPLE PROCESSOR AND A POLYMER-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC COLUMN TO DETERMINE THE PHARMACOKINETICS OF LABETALOL IN MAN
    BATES, J
    CAREY, PF
    GODWARD, RE
    [J]. JOURNAL OF CHROMATOGRAPHY, 1987, 395 : 455 - 461
  • [4] BELLIARDO F, 1991, Giornale Italiano di Chimica Clinica, V16, P107
  • [5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THE ANTIHISTAMINE PYRILAMINE AND ITS N-OXIDE USING ELECTROCHEMICAL DETECTION
    BILLEDEAU, SM
    HOLDER, CL
    GETEK, TA
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 534 : 151 - 159
  • [6] DETERMINATION OF PROPRANOLOL, LABETALOL AND CLENBUTEROL IN RAT-BRAIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    BOTTERBLOM, MHA
    FEENSTRA, MGP
    ERDTSIECKERNSTE, EBHW
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 613 (01): : 121 - 126
  • [7] DETERMINATION OF LABETALOL IN HUMAN-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    DUSCI, LJ
    HACKETT, LP
    [J]. JOURNAL OF CHROMATOGRAPHY, 1979, 175 (01): : 208 - 210
  • [8] HIDALGO IJ, 1985, J CHROMATOGR, V344, P412
  • [9] AUTOMATED SAMPLE PREPARATION ONLINE WITH THERMOSPRAY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE DETERMINATION OF DRUGS IN PLASMA
    LANT, MS
    OXFORD, J
    MARTIN, LE
    [J]. JOURNAL OF CHROMATOGRAPHY, 1987, 394 (01): : 223 - 230
  • [10] MAGUREGUI MI, 1994, 45 ANN M ISE PORT PO