GATA-1 dominantly activates a program of erythroid gene expression in factor-dependent myeloid FDCW2 cells

Erythrocyte development has previously been shown to depend upon the expression of the lineage-restricted trans-acting factor GATA-1, Despite predicted roles for this factor during early development, GATA-1-deficient cells in chimeric mice and embryonic stem cell cultures mature to a late proerythroblast stage and express at least certain genes that normally are thought to be regulated by GATA-1 (including erythroid Kruppel-like factor [EKLF] and the erythropoietin [Epo] receptor), Opportunities to test roles for GATA-1 in erythroid gene activation in these systems therefore are limited, In the present study, in an alternate approach to test the function of GATA-1, GATA-1 has been expressed together with the Epo receptor in myeloid FDCW2 cells and the resulting effects on cytokine-dependent proliferation and erythroid gene expression have been assessed, GATA-1 expression at low levels delayed FDCW2ER cell cycle progression at the G(1) phase specifically during Epo-induced mitogenesis, Upon expression of GATA-1 at increased levels, proliferation in response to Epo, interleukin-3 (IL-3), and stem cell factor was attenuated and endogenous GATA-1, EKLF and beta(maj)-globin gene expression was activated. Friend of GATA-1 (FOG) transcript levels also were enhanced, and ets-1 and c-mpl but not Epo receptor gene expression was induced, Finally, in FDCW2 cells expressing increased levels of GATA-1 and a carboxyl-terminally truncated Epo receptor, Epo (with respect to IL-3 as a control) was shown to markedly promote globin transcript expression. Thus, novel evidence for select hierarchical roles for GATA-1 and Epo in erythroid lineage specification is provided.