Are seminal fluid microorganisms of significance or merely contaminants?

被引:71
作者
Cottell, E [1 ]
Harrison, RF
McCaffrey, M
Walsh, T
Mallon, E
Barry-Kinsella, C
机构
[1] Rotunda Hosp, Royal Coll Surg Ireland, Acad Dept Obstet & Gynaecol, Dublin 12, Ireland
[2] Rotunda Hosp, Human Assisted Reprod Ireland, Dublin 12, Ireland
关键词
semen; urine; microorganisms; leukocytes;
D O I
10.1016/S0015-0282(00)00709-3
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To determine the contribution of urethral and skin flora to seminal fluid cultures and the relation between bacteriospermia and seminal leukocytes. Design: Prospective study. Setting: IVF-ET unit at a university teaching hospital. Patient(s): Sixty men starting an IVF-ET program. Intervention(s): Culture of sequential first-catch urine, midstream urine, and semen samples with evaluation of seminal leukocytes. Main Outcome Measure(s): A comparison of microbes from first-catch urine, midstream urine, and semen samples and the correlations of seminal microbes, elevated leukocyte concentrations, and pregnancy. Result(s): Microoganisms were detected in 37% of first-catch urine samples, 27% of midstream urine samples, and 51% of semen samples. Most microorganisms were gram-positive microbes and were common to both urine and semen samples. Mean and median leukocyte concentrations were 0.98 x 10(6)/mL and 0.10 x 10(6)/mL, respectively. There was no correlation between seminal microbes and raised leukocytes or between leukocytospermia and/or bacteriospermia and pregnancy. Conclusion(s): Microoganisms are commonly found in insignificant quantities in the semen of asymptomatic men. The frequent isolation of gram-positive microbes common to both urine and semen and the absence of a correlation with raised leukocyte concentrations suggest that bacteriospermia most commonly represents contamination. (Fertil Steril(R) 2000;74:465-70. (C) 2000 by American Society for Reproductive Medicine.).
引用
收藏
页码:465 / 470
页数:6
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