Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis

被引:83
作者
Duman, JG [1 ]
Li, N
Verleye, D
Goetz, FW
Wu, DW
Andorfer, CA
Benjamin, T
Parmelee, DC
机构
[1] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
[2] NCI, Div Basic Sci, Expt Carcinogenesis Lab, Bethesda, MD 20892 USA
基金
美国国家科学基金会;
关键词
antifreeze proteins; thermal hysteresis proteins; Dendroides canadensis; insect cold tolerance; freeze avoidance;
D O I
10.1007/s003600050140
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The deduced amino acid sequences of antifreeze proteins (AFPs) from larvae of the beetle Dendroides canadensis were determined from both complementary DNAs (cDNAs) and from peptide sequencing. These consisted of proteins with a 25-residue signal peptide and mature proteins 83 (Dendroides antifreeze protein; DAFP-1) or 84 (DAFP-2) amino acids in length which differed at only two positions. Peptide sequencing yielded sequences which overlapped exactly with those of the deduced cDNA sequences of DAFF-1 and DAFP-2, while the partial sequence of another AFP (DAFP-3) matched 21 of 28 residues. Seven 12- or 13-mer repeating units are present in these antifreeze proteins with a consensus sequence consisting of: Cys-Thr-X-3-Ser-X-5-X-6-Cys-X-8-X-9-Ala-X-11-Thr-X-13, where X-3 and X-11 tend toward charged residues, X-5 tends toward threonine or serine, X-6 toward asparagine or aspartate, X-9 toward asparagine or lysine, and X-13 toward alanine in the 13-mers. The most interesting feature of these proteins is that throughout the length of the mature antifreeze proteins every sixth residue is a cysteine. These sequences are not similar to any of the known fish AFPs, but they are similar to AFPs from the beetle Tenebrio molitor.
引用
收藏
页码:225 / 232
页数:8
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