Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris

被引：249

作者：

Lin-Cereghino, J ^{[1
]}

Wong, WW ^{[1
]}

Xiong, S ^{[1
]}

Giang, W ^{[1
]}

Luong, LT ^{[1
]}

Vu, J ^{[1
]}

Johnson, SD ^{[1
]}

Lin-Cereghino, GP ^{[1
]}

机构：

[1] Univ Pacific, Dept Biol Sci, Stockton, CA 95211 USA

来源：

BIOTECHNIQUES | 2005年 / 38卷 / 01期

关键词：

D O I：

10.2144/05381BM04

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：44 / +

页数：3

共 5 条

[1]

Cereghino JL, 2000, FEMS MICROBIOL REV, V24, P45, DOI 10.1016/S0168-6445(99)00029-7

[2] PICHIA-PASTORIS AS A HOST SYSTEM FOR TRANSFORMATIONS [J].

CREGG, JM ;

BARRINGER, KJ ;

HESSLER, AY ;

MADDEN, KR .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3376-3385

[3]

Cregg JM, 1998, METH MOL B, V103, P27

[4] AN EFFICIENT TRANSFORMATION PROCEDURE ENABLING LONG-TERM STORAGE OF COMPETENT CELLS OF VARIOUS YEAST GENERA [J].

DOHMEN, RJ ;

STRASSER, AWM ;

HONER, CB ;

HOLLENBERG, CP .

YEAST, 1991, 7 (07) :691-692

[5] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].

ITO, H ;

FUKUDA, Y ;

MURATA, K ;

KIMURA, A .

JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :163-168

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