Modelling the hair follicle dermal papilla using spheroid cell cultures

被引：122

作者：

Higgins, Claire A. ^{[1
]}

Richardson, Gavin D. ^{[1
]}

Ferdinando, Dudley ^{[2
]}

Westgate, Gillian E. ^{[3
]}

Jahoda, Colin A. B. ^{[1
]}

机构：

[1] Univ Durham, Sch Biol & Med Sci, Durham DH1 3LE, England

[2] Unilever Discover, Bedford, England

[3] Westgate Consultancy, Bedford, England

来源：

EXPERIMENTAL DERMATOLOGY | 2010年 / 19卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

cell culture; dermal papilla; hair follicle; GROWTH; INVITRO; INDUCTION; IMPLANTATION; ACTIVATION; RESPONSES; VERSICAN; PATHWAY; INVIVO;

D O I：

10.1111/j.1600-0625.2009.01007.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100227 [皮肤病学];

摘要：

Human dermal papilla (DP) cells grown in two-dimensional (2D) culture have been studied extensively. However, key differences exist between DP cell activities in vivo and in vitro. Using a suspension method of cell culture to maintain DP cells, we created three-dimensional (3D) dermal spheres morphologically akin to intact (anagen) DPs. Analysis of these spheres using immunocytochemistry demonstrates that they have expression profiles different from papilla cells cultured in 2D but with many similarities to intact DPs. This method of DP cell culture may provide us with a tool to elucidate our understanding of signalling within the DP as it relates to induction, maintenance or even inhibition of hair growth.

引用

页码：546 / 548

页数：3

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