Silole nanocrystals as novel biolabels

被引：59

作者：

Chan, CPY

Haeussler, M

Tang, BZ

Dong, YQ

Sin, KK

Mak, WC

Trau, D

Seydack, M

Renneberg, R ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Biosensors & Bioelect Lab, Kowloon, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Dept Chem, Inst Nano Mat & Technol, Kowloon, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Bioengn Grad Program, Kowloon, Hong Kong, Peoples R China

[4] 8sens Bionost AG, D-13125 Berlin, Germany

[5] Natl Univ Singapore, Div Bioengn, Singapore 117548, Singapore

[6] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117548, Singapore

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2004年 / 295卷 / 1-2期

关键词：

aggregation-induced emission (AIE); biolabel; fluorescence immunoassay (FIA); layer-by-layer (LbL) technique; silole;

D O I：

10.1016/j.jim.2004.09.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph2Si(CPh)(4); HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4 x 10(3)) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immumoassay using directly fluorescent-labeled antibodies. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：111 / 118

页数：8

共 29 条

[1] Microencapsulation of uncharged low molecular weight organic materials by polyelectrolyte multilayer self-assembly [J].