Oriented immobilization of proteins

被引:205
作者
Rao, SV
Anderson, KW [1 ]
Bachas, LG
机构
[1] Univ Kentucky, Dept Chem, Lexington, KY 40506 USA
[2] Dept Chem & Mat Engn, Lexington, KY 40506 USA
关键词
protein immobilization; site-specific immobilization; site-directed mutagenesis; carbohydrate-mediated immobilization; protein orientation;
D O I
10.1007/BF01243043
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Immobilized enzymes have found numerous applications in analytical, clinical, environmental and industrial chemistry. However, in most cases, immobilization leads to partial or total loss of activity. It is widely believed that the loss in activity is due to attachment of proteins on the immobilization support through several amino acid residues. This results in a random orientation of the immobilized protein and in increased structural deformation due to multi-point attachment. Several researchers have explored ways to orient proteins on surfaces, such that orderly organization, single point attachment and accessibility of the active site (or binding site) are possible. This article reviews the various approaches available to achieve oriented immobilization of proteins and its applications in several disciplines.
引用
收藏
页码:127 / 143
页数:17
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