Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster

被引:73
作者
Toba, G
Aigaki, T
机构
[1] Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo 1920397, Japan
[2] Japan Sci & Technol Corp, Prescursory Res Embryon Sci & Technol, Kyoto 6190237, Japan
关键词
gene search system; oxidative stress; P element excision; pseudogene;
D O I
10.1016/S0378-1119(00)00246-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Microsomal glutathione S-transferase-I (MGST-I) has been thought to be important for protecting the cell from oxidative damages and/or xenobiotics. We have previously identified the Microsomal glutathione S-transferase-like (Mgstl) gene, a Drosophila homologue of human MGST-I. To investigate the function of the enzyme using Diosophila as a model system, we examined the expression pattern of Mgstl during development, and generated loss-of-function mutants to assess its in-vivo function. Mgstl was expressed in all developmental stages. It is expressed ubiquitously with the highest expression in the larval fat body, an insect organ thought to be functionally corresponding to mammalian liver: while relatively low in the central nervous system. This tissue distribution is consistent with that of MGST-I in humans or Rats. Mgstl null mutants generated from a P element insertion line showed no obvious defects in morphology, indicating that it is not essential for the development. However, their life span was significantly reduced compared to control flies, suggesting that the MGSTL protein is involved in processes somehow contributing to aging. We found an Mgstl pseudogene, which is apparently derived through the reverse transcription of Mgstl mRNA and subsequent integration into the genome. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:179 / 187
页数:9
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