The C elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation

被引：26

作者：

Boxem, M

Tsai, CW

Zhang, Y

Saito, RM

Liu, JO

机构：

[1] Johns Hopkins Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[2] Johns Hopkins Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[3] Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA

来源：

FEBS LETTERS | 2004年 / 576卷 / 1-2期

关键词：

map-1; map-2; methionine aminopeptidase; germline development;

D O I：

10.1016/j.febslet.2004.08.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the physiological function of type 2 methionine aminopeptidases (MetAP2) using Caenorhabditis elegans as a model system. A homolog of human MetAP2 was found in the C. elegans genome, which we termed MAP-2. MAP-2 protein displayed methionine aminopeptidase activity and was sensitive to inhibition by fumagillin. Downregulation of map-2 expression by RNAi led to sterility, resulting from a defect in germ cell proliferation. These observations suggest that MAP-2 is essential for germ cell development in C elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：245 / 250

页数：6

相关论文

共 36 条

[1] EUKARYOTIC METHIONYL AMINOPEPTIDASES - 2 CLASSES OF COBALT-DEPENDENT ENZYMES [J].

ARFIN, SM ;

KENDALL, RL ;

HALL, L ;

WEAVER, LH ;

STEWART, AE ;

MATTHEWS, BW ;

BRADSHAW, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :7714-7718

[2] PROCESSING OF THE INITIATION METHIONINE FROM PROTEINS - PROPERTIES OF THE ESCHERICHIA-COLI METHIONINE AMINOPEPTIDASE AND ITS GENE STRUCTURE [J].

BENBASSAT, A ;

BAUER, K ;

CHANG, SY ;

MYAMBO, K ;

BOOSMAN, A ;

CHANG, S .

JOURNAL OF BACTERIOLOGY, 1987, 169 (02) :751-757

[3]

Boxem M, 1999, DEVELOPMENT, V126, P2227

[4] N-terminal processing:: the methionine aminopeptidase and Nα-acetyl transferase families [J].

Bradshaw, RA ;

Brickey, WW ;

Walker, KW .

TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (07) :263-267

[5] METHIONINE AMINOPEPTIDASE GENE OF ESCHERICHIA-COLI IS ESSENTIAL FOR CELL-GROWTH [J].

CHANG, SYP ;

MCGARY, EC ;

CHANG, S .

JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :4071-4072

[6] A methionine aminopeptidase and putative regulator of translation initiation is required for cell growth and patterning in Drosophila [J].

Cutforth, T ;

Gaul, U .

MECHANISMS OF DEVELOPMENT, 1999, 82 (1-2) :23-28

[7]

DATTA B, 1989, J BIOL CHEM, V264, P20620

[8] ROLES OF A 67-KDA POLYPEPTIDE IN REVERSAL OF PROTEIN-SYNTHESIS INHIBITION IN HEME-DEFICIENT RETICULOCYTE LYSATE [J].

DATTA, B ;

CHAKRABARTI, D ;

ROY, AL ;

GUPTA, NK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) :3324-3328

[9] A glycosylation site, 60SGTS63, of p67 is required for its ability to regulate the phosphorylation and activity of eukaryotic initiation factor 2α [J].

Datta, R ;

Choudhury, P ;

Ghosh, A ;

Datta, B .

BIOCHEMISTRY, 2003, 42 (18) :5453-5460

[10] Protection of translation initiation factor eIF2 phosphorylation correlates with eIF2-associated glycoprotein p67 levels and requires the lysine-rich domain I of p67 [J].

Datta, R ;

Choudhury, P ;

Bhattacharya, M ;

Leon, FS ;

Zhou, Y ;

Datta, B .

BIOCHIMIE, 2001, 83 (10) :919-931

← 1 2 3 4 →