Early-warning electrochemical biosensor system for environmental monitoring based on enzyme inhibition

被引：51

作者：

Dzyadevych, SV

Soldatkin, AP

Arkhypova, VN

El'skaya, AV

Chovelon, JM

Georgiou, CA

Martelet, C

Jaffrezic-Renault, N

机构：

[1] Ecole Cent Lyon, CEGELY, UMR 5005, F-69134 Ecully, France

[2] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Lab Biomol Elect, UA-03143 Kiev, Ukraine

[3] Univ Lyon 1, CNRS, Lab Applicat Chim Envieonm, F-69622 Villeurbanne, France

[4] Agr Univ Athens, Chem Lab, Athens 11855, Greece

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 105卷 / 01期

关键词：

electrochemical enzyme biosensor; cholinesterases; organophosphorous and carbamate pesticides; enzyme inhibition mechanism;

D O I：

10.1016/j.snb.2004.02.039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Potentiometric and conductometric biosensors based on immobilised cholinesterases have been developed. Detection of some organophosphorous pesticides (methyl parathion, methyl paraoxon, ethyl paraoxon, trichlorphon, diisopropyl fluorophosphates) and carbamate pesticide (carbofuran) have been realised through enzyme inhibition mechanisms. Comparison of sensitivity of the biosensor systems developed with that of traditional analytical techniques (high performance liquid chromatography and Lumistox test based on Vibrio fischeri luminescent bacteria) has shown that biosensor measuring procedures are well suited for fast environmental control. Analytical characteristics of the systems created, their advantages and shortcomings are discussed. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：81 / 87

页数：7

共 19 条

[1] Multibiosensor based on enzyme inhibition analysis for determination of different toxic substances [J].