Functional association between CBP and HNF4 in trans-activation

被引：102

作者：

Yoshida, E

Aratani, S

Itou, H

Miyagishi, M

Takiguchi, M

Osumu, T

Murakami, K

Fukamizu, A ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Appl Biochem, Ibaraki, Osaka 305, Japan

[2] Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Ibaraki, Osaka 305, Japan

[3] Kumamoto Univ, Sch Med, Dept Mol Genet, Kumamoto 860, Japan

[4] Himeji Inst Technol, Fac Sci, Dept Life Sci, Himeji, Hyogo 67122, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 241卷 / 03期

基金：

日本学术振兴会;

关键词：

CBP; HNF4; transcription; gene expression; promoter; transcription factor; coactivator;

D O I：

10.1006/bbrc.1997.7871

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CBP functions as a key transcriptional coactivator for a variety of transcription factors. We show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP as a bait. GST-pull down assay with nuclear extracts or in vitro translation products revealed that CBP and HNF4 interact with each other at the middle portion (aa 119-375) of HNF4 and two distinct regions (aa 271-451 and 1626-2259) of CBP, respectively, in the ligand-independent manner. Cotransfection experiments indicated that CBP is capable of activating HNF4 site-mediated transcription. These results suggested a functional association between CBP and HNF4 in trans-activation. (C) 1997 Academic Press.

引用

页码：664 / 669

页数：6

共 22 条

[1] E1A-ASSOCIATED P300 AND CREB-ASSOCIATED CBP BELONG TO A CONSERVED FAMILY OF COACTIVATORS [J].