Ectopic Expression of the Tomato Mi-1 Gene Confers Resistance to Root Knot Nematodes in Lettuce (Lactuca sativa)

被引:15
作者
Zhang, Li-Ying [1 ,2 ]
Zhang, Yu-Yang [1 ]
Chen, Ru-Gang [1 ]
Zhang, Jun-Hong [1 ]
Wang, Tao-Tao [1 ]
Li, Han-Xia [1 ]
Ye, Zhi-Biao [1 ]
机构
[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
[2] Beijing Vegetable Res Ctr, Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China
关键词
Ectopic expression; Mi-1; Nematode resistance; Lactuca sativa; MELOIDOGYNE-INCOGNITA;
D O I
10.1007/s11105-009-0143-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The full genomic region of the root knot nematode (Meloidogyne spp.) resistance gene Mi-1 was cloned from tomato and transformed into lettuce to investigate its function in a heterologous system. Transgenic lettuce lines containing the Mi-1 gene were developed using Agrobacterium-mediated transformation. Ectopic expression of the Mi-1 gene was observed in transgenic lines, and resistance to root knot nematode was improved.
引用
收藏
页码:204 / 211
页数:8
相关论文
共 26 条
[1]   The heat-stable root-knot nematode resistance gene Mi-9 from Lycopersicon peruvianum is localized on the short arm of chromosome 6 [J].
Ammiraju, JSS ;
Veremis, JC ;
Huang, X ;
Roberts, PA ;
Kaloshian, I .
THEORETICAL AND APPLIED GENETICS, 2003, 106 (03) :478-484
[2]   RNA interference and plant parasitic nematodes [J].
Bakhetia, M ;
Charlton, WL ;
Urwin, PE ;
McPherson, MJ ;
Atkinson, HJ .
TRENDS IN PLANT SCIENCE, 2005, 10 (08) :362-367
[3]   CaMi, a root-knot nematode resistance gene from hot pepper (Capsium annuum L.) confers nematode resistance in tomato [J].
Chen, Rugang ;
Li, Hanxia ;
Zhang, Liying ;
Zhang, Junhong ;
Xiao, Jinghua ;
Ye, Zhibiao .
PLANT CELL REPORTS, 2007, 26 (07) :895-905
[4]   Plant pathogens and integrated defence responses to infection [J].
Dangl, JL ;
Jones, JDG .
NATURE, 2001, 411 (6839) :826-833
[5]   Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome [J].
Djian-Caporalino, C. ;
Fazari, A. ;
Arguel, M. J. ;
Vernie, T. ;
VandeCasteele, C. ;
Faure, I. ;
Brunoud, G. ;
Pijarowski, L. ;
Palloix, A. ;
Lefebvre, V. ;
Abad, P. .
THEORETICAL AND APPLIED GENETICS, 2007, 114 (03) :473-486
[6]   CURRENT STATUS OF GENE-FOR-GENE CONCEPT [J].
FLOR, HH .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1971, 9 :275-+
[7]  
Funk V. A., 2005, Plant diversity and complexity patterns: local, regional and global dimensions. Proceedings of an International Symposium held at the Royal Danish Academy of Sciences and Letters in Copenhagen, Denmark, 25-28 May, 2003, P343
[8]   Heterologous expression of the Mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant [J].
Goggin, FL ;
Jia, LL ;
Shah, G ;
Hebert, S ;
Williamson, VM ;
Ullman, DE .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2006, 19 (04) :383-388
[9]   Instability of Mi-mediated nematode resistance in transgenic tomato plants [J].
Goggin, FL ;
Shah, G ;
Williamson, VM ;
Ullman, DE .
MOLECULAR BREEDING, 2004, 13 (04) :357-364
[10]   Inheritance of the resistant reaction of the lettuce cultivar 'Grand Rapids' to the southern root-knot nematode Meloidogyne incognita (Kofoid & White) Chitwood [J].
Gomes, LAA ;
Maluf, WR ;
Campos, VP .
EUPHYTICA, 2000, 114 (01) :37-46