Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I

被引：38

作者：

Jakoby, M ^{[1
]}

Tesch, M ^{[1
]}

Sahm, H ^{[1
]}

Kramer, R ^{[1
]}

Burkovski, A ^{[1
]}

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, INST BIOTECHNOL 1, D-52425 JULICH, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 154卷 / 01期

关键词：

Corynebacterium; nitrogen metabolism; glutamine synthetase; glnA;

D O I：

10.1016/S0378-1097(97)00304-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.

引用

页码：81 / 88

页数：8

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