Soil molecular microbial ecology at age 20: methodological challenges for the future

被引:48
作者
Ogram, A
机构
[1] Univ Florida, Dept Soil & Water Sci, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
关键词
methods; molecular ecology; soil DNA;
D O I
10.1016/S0038-0717(00)00088-2
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
The year 2000 marks the twentieth anniversary of the publication in Soil Biology & Biochemistry by Vigdis L. Torsvik (University of Bergen) of the first procedure for isolation of bacterial DNA from soil (Torsvik, 1980), arguably initiating the subdiscipline of soil molecular microbial ecology. Since 1980, great strides have been made in the development of methods and in the application of genetic tools to analysis of soil microbial communities, and many soil microbiology laboratories routinely incorporate these tools in their research. IT is likely that the concept of soil molecular ecology will soon disappear as a subdiscipline of microbial ecology, and that these tools will become as routine and indispensable as are genetic tools in microbial physiology. However, even though increasing numbers of soil microbiologists use molecular biology in their research, some fundamental obstacles must be overcome before these tools become as routine as are, for example, many soil chemical methods. This anniversary provides an opportunity for retrospection on the applicability of genetic tools to soil microbial ecology, and of methodological needs for the immediate future. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1499 / 1504
页数:6
相关论文
共 49 条
[1]   REPEATED SEQUENCES IN DNA [J].
BRITTEN, RJ ;
KOHNE, DE .
SCIENCE, 1968, 161 (3841) :529-&
[2]   Automated nucleic acid isolation and purification from soil extracts using renewable affinity microcolumns in a sequential injection system [J].
Chandler, DP ;
Schuck, BL ;
Brockman, FJ ;
Bruckner-Lea, CJ .
TALANTA, 1999, 49 (05) :969-983
[3]   Redefining relativity: quantitative PCR at low template concentrations for industrial and environmental microbiology [J].
Chandler, DP .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 21 (03) :128-140
[4]   Depth profile of sulfate-reducing bacterial ribosomal RNA and mercury methylation in an estuarine sediment [J].
Devereux, R ;
Winfrey, MR ;
Winfrey, J ;
Stahl, DA .
FEMS MICROBIOLOGY ECOLOGY, 1996, 20 (01) :23-31
[5]   EFFECT OF GENOME SIZE AND RRN GENE COPY NUMBER ON PCR AMPLIFICATION OF 16S RIBOSOMAL-RNA GENES FROM A MIXTURE OF BACTERIAL SPECIES [J].
FARRELLY, V ;
RAINEY, FA ;
STACKEBRANDT, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2798-2801
[6]  
Felske A, 1998, APPL ENVIRON MICROB, V64, P4581
[7]   Direct ribosome isolation from soil to extract bacterial rRNA for community analysis [J].
Felske, A ;
Engelen, B ;
Nubel, U ;
Backhaus, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) :4162-4167
[8]   QUANTITATIVE RELATIONSHIP BETWEEN NAPHTHALENE CATABOLIC GENE-FREQUENCY AND EXPRESSION IN PREDICTING PAH DEGRADATION IN SOILS AT TOWN GAS MANUFACTURING SITES [J].
FLEMING, JT ;
SANSEVERINO, J ;
SAYLER, GS .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (06) :1068-1074
[9]   Prokaryotic genome size and SSU rDNA copy number: Estimation of microbial relative abundance from a mixed population [J].
Fogel, GB ;
Collins, CR ;
Li, J ;
Brunk, CF .
MICROBIAL ECOLOGY, 1999, 38 (02) :93-113
[10]   DETERMINATION OF MOLECULAR WEIGHT OF BACTERIAL GENOME DNA FROM RENATURATION RATES [J].
GILLIS, M ;
DELEY, J ;
DECLEENE, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 12 (01) :143-&