Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter

被引：40

作者：

Lange, C ^{[1
]}

Walther, W ^{[1
]}

Schwabe, H ^{[1
]}

Stein, U ^{[1
]}

机构：

[1] Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 278卷 / 01期

关键词：

multidrug resistance; human major vault protein/lung resistance-related protein; gene promoter;

D O I：

10.1006/bbrc.2000.3782

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lung resistance-related protein (LRP) was identified as the human major vault protein (MVP), and is overexpressed in various multidrug-resistant cancer cell lines and clinical samples. We characterized DNA sequences upstream to the transcription initiation site of the MVP gene in the human non-small cell lung cancer cell line SW-1573. A 1.9-kb and a shortened 0.7-kb fragment of the 5'-upstream genomic: region show strong promoter activity in chloramphenicol acetyltransferase (CAT) reporter assays. The promoter is TATA-less and contains an inverted CCAAT: box and a Spl site located near to a p53 binding motif. An alternative 3'-splice site of intron 1 results in a splicing variant within the 5'-untranslated region of MVP mRNA. (C) 2000 Academic Press.

引用

收藏

页码：125 / 133

页数：9

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