DNA optical detection based on porous silicon technology: from biosensors to biochips

被引：75

作者：

De Stefano, Luca

Arcari, Paolo

Lamberti, Annalisa

Sanges, Carmen

Rotiroti, Lucia

Rea, Ilaria

Rendina, Ivo

机构：

[1] Univ Naples, Inst Microelect & Microsyst, Unit Naples, Natl Res Council, I-80131 Naples, Italy

[2] Univ Naples Federico II, Dept Biochem & Med Biotechnol, I-80131 Naples, Italy

[3] Univ Naples Federico II, Dept Organ Chem & Biochem, I-80128 Naples, Italy

[4] Univ Naples Federico II, Dept Phys Sci, I-80126 Naples, Italy

来源：

SENSORS | 2007年 / 7卷 / 02期

关键词：

optical biosensors; porous silicon; biochip;

D O I：

10.3390/s7020214

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 mu M range has been realized.

引用

页码：214 / 221

页数：8

共 11 条

[1] A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface [J].