Human NRD convertase: A highly conserved metalloendopeptidase expressed at specific sites during development and in adult tissues

被引:33
作者
Fumagalli, P
Accarino, M
Egeo, A
Scartezzini, P
Rappazzo, G
Pizzuti, A
Avvantaggiato, V
Simeone, A
Arrigo, G
ZUffardi, O
Ottolenghi, S
Taramelli, R
机构
[1] Univ Milan, Dipartimento Genet & Biol Microrganismi, I-20133 Milan, Italy
[2] EO Osped Galliera, Div Pediat, Genoa, Italy
[3] Dipartimento Biol Anim, Catania, Italy
[4] Osped Policlin, Neurol Clin, Milan, Italy
[5] IRCCS, CSS, S Giovanni Rotondo, Italy
[6] CNR, Ist Int Genet & Biofis, I-80125 Naples, Italy
[7] Biol Gen & Genet Med, Pavia, Italy
[8] Osped S Raffaele, Lab Citogenet, Milan, Italy
关键词
D O I
10.1006/geno.1997.5078
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We report the cloning of the human homologue of the rat metalloprotease N-arginine dibasic convertase (NRD convertase). This endopeptidase is responsible for the processing, at the Arg-Lys dibasic site on the N-terminal side of the arginine residue, of propeptides and proproteins. Comparisons of the human and rat full-length cDNAs show similarity and identity of 94 and 91%, respectively. In humans NRD convertase is predominantly expressed in heart, skeletal muscle, and testis. We have also studied the expression of this gene in mouse at various developmental stages and found that the neural tissue is the almost exclusive site of expression in early development (between E 10.5 and E 16.5). To gain information about the possibility that defects in this gene are linked to inherited neuromuscular disorders, we determined the chromosomal location of the human NRD convertase gene by FISH analysis, showing that the gene resides at 1p32.2. (C) 1998 Academic Press.
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页码:238 / 245
页数:8
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