Application of flow cytometry for rapid determination of cell number of viable bacteria

被引：15

作者：

Endo, H ^{[1
]}

Nakayama, J ^{[1
]}

Hayashi, T ^{[1
]}

Watanabe, E ^{[1
]}

机构：

[1] Tokyo Univ Fisheries, Dept Food Sci & Technol, Tokyo 108, Japan

来源：

FISHERIES SCIENCE | 1997年 / 63卷 / 06期

关键词：

flow cytometry; bacteria; cell counts; fluorescein diacetate;

D O I：

10.2331/fishsci.63.1024

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The application of the Flow cytometry (FCM) technique for rapid determination of cell number of viable bacteria suspended in seawater is described. A fluorescein diacetate was used to distinguish between viable cells and dead cells for various bacteria. The FCM technique was also used to discriminate between Gram-positive and Gram-negative bacteria using the LIVE BacLight(TM) Bacterial gram stain kit. A difference in the fluorescence scattergram between the two bacterial groups was observed. The FCM method provided rapid determination of cell number of viable bacteria in seawater. A good correlation was observed between the values determined by the FCM method and the colony counting method in the range of 10(5)-10(9) cells/ml. One FCM assay could be completed within 60 s and the total assay time including the preparation of bacteria was within 35 min.

引用

页码：1024 / 1029

页数：6

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