Co-stimulation of human breast cancer cells with transforming growth factor-β and tenascin-C enhances matrix metalloproteinase-9 expression and cancer cell invasion

被引：36

作者：

Ilunga, K

Nishiura, R

Inada, H

El-Karef, A

Imanaka-Yoshida, K

Sakakura, T

Yoshida, T ^{[1
]}

机构：

[1] Mie Univ, Sch Med, Dept Pathol, 2-174 Edobashi, Tsu, Mie 5148507, Japan

[2] Lubumbashi Univ, Fac Med, Dept Surg, Lubumbashi, DEM REP CONGO

来源：

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 2004年 / 85卷 / 06期

关键词：

breast cancer; cancer cell invasion; matrix metalloproteinase-9; tenascin-C; transforming growth factor-beta;

D O I：

10.1111/j.0959-9673.2004.00406.x

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Transforming growth factor-beta (TGF-beta), tenascin-C (TN-C) and matrix metalloproteinases (MMPs) have been demonstrated independently to be associated with disease progression and poor prognosis in patients with breast cancer. The present study explored effects of TGF-beta and TN-C on MMP-9 expression and cancer invasion. An experimental study was designed to analyse MDA-MB-231 breast cancer cells, known for their high invasiveness, after stimulation with TGF-beta1 and/or TN-C. TGF-beta1 stimulated TN-C expression in the cells. Co-stimulation of MDA-MB-231 cells with TN-C and TGF-beta increased MMP-9 expression at both the gene (28-fold) and the protein levels. The in vitro invasion also increased (4-fold). GM6001 inhibited the invasion induced by the co-stimulation. The combined effect of TN-C and TGF-beta resulted in enhanced MMP-9 expression and cancer invasion in vitro.

引用

页码：373 / 379

页数：7

共 42 条

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