Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein

被引：15

作者：

Crescenzi, A

d'Aquino, L

Nuzzaci, M

Ostuni, A

Bavoso, A

Comes, S

De Stradis, A

Piazzolla, P

机构：

[1] Univ Basilicata, Dipartimento Biol Difesa & Biotecnol Agro Foresta, I-85100 Potenza, Italy

[2] Univ Basilicata, Dipartimento Chim, I-85100 Potenza, Italy

[3] Univ Basilicata, Ctr Serv Interdipartimentali Microscopia Elettr, I-85100 Potenza, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 69卷 / 1-2期

关键词：

strain specific polyclonal antiserum; plum pox potyvirus; synthetic polypeptide; nucleotide sequence;

D O I：

10.1016/S0166-0934(97)00158-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests. (C) 1997 Elsevier Science B.V.

引用

页码：181 / 189

页数：9

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