Molecular analysis of SCARECROW genes expressed in white lupin cluster roots

被引:29
作者
Sbabou, Laila [1 ,2 ]
Bucciarelli, Bruna [3 ]
Miller, Susan [3 ]
Liu, Junqi [1 ]
Berhada, Fatiha [2 ]
Filali-Maltouf, Abdelkarim [2 ]
Allan, Deborah [4 ]
Vance, Carroll [1 ,3 ]
机构
[1] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA
[2] Univ Mohammed 5, Fac Sci, Lab Microbiol & Biol Mol, Rabat, Morocco
[3] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA
[4] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA
基金
美国农业部;
关键词
Cluster roots; endodermis; interfering RNA; Lupinus albus; scarecrow; short roots; PROTEOID ROOTS; PHOSPHORUS DEFICIENCY; ENHANCED EXPRESSION; PHOSPHATE STARVATION; RADIAL ORGANIZATION; ARABIDOPSIS; REVEALS; PATTERN; ALBUS; IDENTIFICATION;
D O I
10.1093/jxb/erp400
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Scarecrow (SCR) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent centre and differentiation of the endodermis. In response to phosphorus (P) deficiency, white lupin (Lupinus albus L.) root surface area increases some 50-fold to 70-fold due to the development of cluster (proteoid) roots. Previously it was reported that SCR-like expressed sequence tags (ESTs) were expressed during early cluster root development. Here the cloning of two white lupin SCR genes, LaSCR1 and LaSCR2, is reported. The predicted amino acid sequences of both LaSCR gene products are highly similar to AtSCR and contain C-terminal conserved GRAS family domains. LaSCR1 and LaSCR2 transcript accumulation localized to the endodermis of both normal and cluster roots as shown by in situ hybridization and gene promoter::reporter staining. Transcript analysis as evaluated by quantitative real-time-PCR (qRT-PCR) and RNA gel hybridization indicated that the two LaSCR genes are expressed predominantly in roots. Expression of LaSCR genes was not directly responsive to the P status of the plant but was a function of cluster root development. Suppression of LaSCR1 in transformed roots of lupin and Medicago via RNAi (RNA interference) delivered through Agrobacterium rhizogenes resulted in decreased root numbers, reflecting the potential role of LaSCR1 in maintaining root growth in these species. The results suggest that the functional orthologues of AtSCR have been characterized.
引用
收藏
页码:1351 / 1363
页数:13
相关论文
共 47 条
[1]   The role of GRAS proteins in plant signal transduction and development [J].
Bolle, C .
PLANTA, 2004, 218 (05) :683-692
[2]  
Chabaud M, 2006, MEDICAGO TRUNCATULA
[3]  
CUI H, 2007, SCIENCE, V316, P20
[4]   The SCARECROW gene regulates an asymmetric cell division that is essential for generating the radial organization of the Arabidopsis root [J].
DiLaurenzio, L ;
WysockaDiller, J ;
Malamy, JE ;
Pysh, L ;
Helariutta, Y ;
Freshour, G ;
Hahn, MG ;
Feldmann, KA ;
Benfey, PN .
CELL, 1996, 86 (03) :423-433
[5]  
Dinkelaker Barbara, 1995, Botanica Acta, V108, P183
[6]   Proteoid root development of phosphorus deficient lupin is mimicked by auxin and phosphonate [J].
Gilbert, GA ;
Knight, JD ;
Vance, CP ;
Allan, DL .
ANNALS OF BOTANY, 2000, 85 (06) :921-928
[7]   Identification of candidate phosphorus stress induced genes in Phaseolus vulgaris through clustering analysis across several plant species [J].
Graham, Michelle A. ;
Ramirez, Mario ;
Valdes-Lopez, Oswaldo ;
Lara, Miguel ;
Tesfaye, Mesfin ;
Vance, Carroll P. ;
Hernandez, Georgina .
FUNCTIONAL PLANT BIOLOGY, 2006, 33 (08) :789-797
[8]   Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants [J].
Hammond, JP ;
Bennett, MJ ;
Bowen, HC ;
Broadley, MR ;
Eastwood, DC ;
May, ST ;
Rahn, C ;
Swarup, R ;
Woolaway, KE ;
White, PJ .
PLANT PHYSIOLOGY, 2003, 132 (02) :578-596
[9]   Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division [J].
Heidstra, R ;
Welch, D ;
Scheres, B .
GENES & DEVELOPMENT, 2004, 18 (16) :1964-1969
[10]   High-throughput vectors for efficient gene silencing in plants [J].
Helliwell, CA ;
Wesley, SV ;
Wielopolska, AJ ;
Waterhouse, PM .
FUNCTIONAL PLANT BIOLOGY, 2002, 29 (10) :1217-1225