Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2)

被引：102

作者：

Collet, B

Hovens, GCJ

Mazzoni, D

Hirono, I

Aoki, T

Secombes, CJ

机构：

[1] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Dept Zool, Aberdeen AB24 2TZ, Scotland

[2] Tokyo Univ Fisheries, Dept Aquat Biosci, Tokyo 108, Japan

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2003年 / 27卷 / 02期

关键词：

Interferon regulatory factor 1; Interferon regulatory factor 2; rainbow trout; rainbow trout gonad; DNA vaccination; viral haemorrhagic septicaemia virus;

D O I：

10.1016/S0145-305X(02)00072-1

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 [水产];

摘要：

Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) I and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145 bp and a 3' UTR of 481 bp. The IRF-2 cDNA contains a 1035 bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146 bp and a 3' UTR of 925 bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection I week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：111 / 126

页数：16

共 38 条

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