High-dose immune suppression and autologous hematopoietic stem cell transplantation in refractory Crohn disease

Two patients with severe Crohn disease, defined by a Crohn Disease Activity Index (CDAI) higher than 250 despite anti-tumor necrosis factor alpha (TNF-alpha), were treated by intense immune suppression and autologous hematopoietic stem cell transplantation (HSCT). Stem cells were mobilized from the peripheral blood using cyclophosphamide (2.0 g/m(2)) and granulocyte colony-stimulating factor (G-CSF; 5 mug/kg/d), enriched ex vivo, by CD34(+) selection, and reinfused after immune conditioning with cyclophosphamide (200 mg/kg) and equine anti-thymocyte globulin (ATG; 90 mg/kg). Patients have remained in remission (CDAI < 100) for 1 year since HSCT We conclude that further HSCT studies for severe Crohn disease appear warranted.