Subtilisin catalysis of substrate anchored in cyclodextrin

被引：2

作者：

Prabhu, KS ^{[1
]}

Ramadoss, CS ^{[1
]}

机构：

[1] Vittal Mallya Sci Res Fdn, Div Biol Sci, Bangalore 560004, Karnataka, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 69卷 / 01期

关键词：

anchored substrate; cyclodextrin; subtilisin; catalysis;

D O I：

10.1007/BF02786022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The N-benzoyl-L-tyrosine ethyl ester (BTEE) anchored in cyclodextrin (beta and beta-methyl CD) serves as an excellent substrate for subtilisin Carlsberg catalysis. The rate of hydrolysis was found to be approximately twofold higher than that of the methanolic substrate. The K-mapp and V-max values for the CD anchored substrates were significantly higher than the methanol-solubilized BTEE.

引用

页码：69 / 77

页数：9

共 26 条

[1]

BIASE DD, 1996, EUR J BIOCHEM, V237, P93

[2] ALTERATION OF THE SPECIFICITY OF SUBTILISIN BPN' BY SITE-DIRECTED MUTAGENESIS IN ITS S1 AND S1' BINDING-SITES [J].

BONNEAU, PR ;

GRAYCAR, TP ;

ESTELL, DA ;

JONES, JB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (03) :1026-1030

[3]

BOSSHARD H R, 1974, Biochemistry, V13, P266, DOI 10.1021/bi00699a006

[4]

BRANDEN C, 1992, INTRO PROTEIN STRUCT, P231

[5]

CHEN JP, 1989, BIOTECHNOL LETT, V2, P633

[6]

COOK HW, 1975, CAN J BIOCHEM CELL B, V53, P1220, DOI 10.1139/o75-167

[7] CYCLIC-PEPTIDES AS PROTEASES - A REEVALUATION [J].

COREY, DR ;

PHILLIPS, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4106-4109

[8] INFLUENCE OF GEOMETRIC PROPERTIES OF ACTIVE CENTER ON SPECIFICITY OF ALPHA-CHYMOTRYPSIN CATALYSIS [J].

DOROVSKA, VN ;

MARTINEK, K ;

KAZANSKA.NF ;

KLYOSOV, AA ;

VARFOLOM.SD .

FEBS LETTERS, 1972, 23 (01) :122-&

[9]

Gerhartz W., 1990, Enzymes in industry : production and applications

[10]

GRON H, 1992, BIOCHEMISTRY-US, V31, P8967, DOI 10.1021/bi00152a037

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