Development of an HPLC method for the determination of doripenem in human and mouse serum

被引：26

作者：

Sutherland, Christina

Nicolau, David P.

机构：

[1] Hartford Hosp, Ctr Anti Infect Res & Dev, Ishikari, Hokkaido 06102, Japan

[2] Hartford Hosp, Div Infect Dis, Hartford, CT 06115 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 853卷 / 1-2期

关键词：

doripenern; HPLC; murine; human;

D O I：

10.1016/j.jchromb.2007.03.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC method utilizing solid phase extraction was developed to analyze doripenem, (formerly S-4661) in human and mouse serum. A reversed-phase column was used with a UV detector set at 295 nm. The mobile phase consisted of methanol and phosphate buffer at a flow rate of 1.5 ml/min. Meropenem was used as the internal standard. The standard curve was linear over a range of 0.5-40 mu g/ml. The assay is simple, reproducible, and accurate and has been used successfully to analyze doripenem concentrations from a murine pharmacokinetic study. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：123 / 126

页数：4

共 4 条

[1] In vitro activity of doripenem (S-4661) against multidrug-resistant gram-negative bacilli isolated from patients with cystic fibrosis [J].