Subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult Sudanese population using miswak or toothbrush regularly

Little information is available on the effect of miswak use on gingival microbiota. We assessed levels of 28 oral bacteria in subgingival plaque of adult Sudanese miswak (n=38) and toothbrush users (n=36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. A pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. Whole genomic DNA probes and the checkerboard DNA-DNA hybridization were used in assessing 74 pooled samples. Using 105 bacterial cells threshold, between 2.6% and 47.4% of miswak users and between 2.8% and 36.1% of toothbrush users harbored the investigated species. The percentages of subjects with the investigated species at 106 bacterial cells varied between 2.6% and 39.5% in miswak and between 2.8% and 36.1% in toothbrush users. Miswak users harbored significantly higher Streptococcus intermedius, Actinobacillus actinomycetemcomitans, Veillonella parvula, Actinomyces israelii, and Capnocytophaga gingivalis, and significantly lower Selenomonas sputigena, Streptococcus salivarius, Actinomyces naeslundii, and Streptococcus oralis than did toothbrush users. Probing pocket depth >6 mm showed significantly (P<0.05) higher levels of Porphyromonas gingivalis, Treponema denticola, Bacteroides forsythus, Fusobacterium nucleatum, and V parvula than those 4-5 mm. Our results indicate that the type of oral hygiene had a significant effect on levels of 11 out of 28 bacterial species, and that the type of effect was also dependent on type of bacteria and probing pocket depth.