Sclerostin binds and regulates the activity of cysteine-rich protein 61

被引：22

作者：

Craig, Theodore A. ^{[1
]}

Bhattacharya, Resham ^{[2
]}

Mukhopadhyay, Debabrata ^{[2
]}

Kumar, Rajiv ^{[1
,2
]}

机构：

[1] Mayo Clin, Div Nephrol & Hypertens, Dept Med, Rochester, MN 55905 USA

[2] Mayo Clin, Dept Biochem & Mol Biol, Rochester, MN 55905 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 392卷 / 01期

关键词：

Sclerostin; Cyr61; CCN1; Osteoblasts; Osteoclasts; High bone mass; LRP5; Wnt; HIGH-BONE MASS; MORPHOGENETIC PROTEIN; LRP5; MUTATIONS; BMP ANTAGONIST; CCN FAMILY; CELLS; SOST; DENSITY; DIFFERENTIATION; OSTEOBLASTS;

D O I：

10.1016/j.bbrc.2009.12.143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：36 / 40

页数：5

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