Parylene-A coated microplate for covalent immobilization of proteins and peptides

被引：34

作者：

Jeon, Byung-Jin ^{[1
]}

Kim, Moo-Hwan ^{[2
]}

Pyun, Jae-Chul ^{[1
]}

机构：

[1] Yonsei Univ, Dept Mat Sci & Engn, Seoul 120749, South Korea

[2] Femtoscience LTD, Hwa Seong Si, Gyeong Gi Do, South Korea

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 353卷 / 1-2期

关键词：

Immunoassay; Peptide; Covalent immobilization; Parylene;

D O I：

10.1016/j.jim.2009.11.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：44 / 48

页数：5

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