Development of an enzyme-linked immunosorbent assay for the fungicide imazalil in citrus fruits

被引：27

作者：

Watanabe, E

Watanabe, S

Ito, S

Hayashi, M

Watanabe, T

Yuasa, Y

Nakazawa, H

机构：

[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan

[2] Environm Immunochem Technol Co, Satte Off, Satte, Saitama 3400193, Japan

[3] Environm Immunochem Technol Co Ltd, Shizuoka Off, Kikukawa, Shizuoka 4390031, Japan

[4] Environm Immunochem Technol Co Ltd, Head Off, Minato Ku, Tokyo 1050013, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 11期

关键词：

ELISA; imazalil; monoclonal antibody; citrus fruit; HPLC;

D O I：

10.1021/jf000055k

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Imazalil has been widely used in citrus fruits such as lemons, oranges, and grapefruits.. A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of residual imazalil in citrus fruits. A monoclonal antibody (MoAb) generated to the synthetic imazalil hapten (EIT-0073)-protein conjugate was used. This assay was applied to lemon, orange, and grapefruit matrices for an imazalil analysis. The acceptable residue level for lemons, oranges, and grapefruits in Japan is 5 ppm. The matrix interference was minimized by direct dilution of the sample homogenate. No further cleanup was needed. The detection limit for imazalil in these citrus fruits was 0.1 ng/mL. The recovery of each fortified citrus fruit sample was > 81.0%. The imazalil recovery measured by the proposed ELISA was compared to the recovery determined by a conventional HPLC. A good correlation was observed between the proposed ELISA and the HPLC. This proposed ELISA would be useful for monitoring for residual imazalil.

引用

页码：5124 / 5130

页数：7

共 31 条

[1] Determination of carbaryl, carbofuran and methiocarb in cucumbers and strawberries by monoclonal enzyme immunoassays and high-performance liquid chromatography with fluorescence detection -: An analytical comparison [J].